Journal: Targeted Oncology

Article Title: Absorption, Distribution, and Binding Profile of ME-401, a Potent and Selective Oral Small-Molecule Inhibitor of Phosphatidylinositol 3-Kinase δ (PI3Kδ) in Animal and B-Cell Lymphoma Models

doi: 10.1007/s11523-019-00668-y

Figure Lengend Snippet: Binding kinetics of ME-401 and idelalisib on PIK3CD/PIK3R1

Article Snippet: Kinase protein immobilization: An aliquot of biotinylated PIK3CD/PIK3R1 (Carna Biosciences, Inc., Kobe, Japan) was thawed, diluted to 40 µg/mL in precooled Biacore buffer (50 mM Tris pH 7.5, 0.05% Tween, 150 mM NaCl, and 5 mM MgCl 2 ), and immobilized with the biotin tag on a Series S Sensor Chip SA.

Techniques: Binding Assay

Journal: PLoS ONE

Article Title: Molecular Characterization of Notch1 Positive Progenitor Cells in the Developing Retina

doi: 10.1371/journal.pone.0131054

Figure Lengend Snippet: A) The purity of isolated cells was tested using anti-Notch1 antibody (green). Propidium iodide (PI, red) was used to visualize the nucleus of the cell. Bar is 50 μm. B) The levels of Notch1 expression in isolated cells were higher compared to expression of Notch1 in whole retinas. Gene expression was measured by qRT-PCR. Results are expressed as a percentage of the corresponding value in the Notch1 + cells isolated from embryonic day 14 developing retinas ± SEM (*P < 0.05).

Article Snippet: To isolate Notch1 + cells, we used monoclonal biotin conjugated anti-Notch1 mouse antibodies (130-096-557, Miltenyi Biotec, Auburn, CA) and followed a protocol for immunomagnetic separation provided by Miltenyi Biotec (Auburn, CA).

Techniques: Isolation, Expressing, Gene Expression, Quantitative RT-PCR

Journal: PLoS ONE

Article Title: Molecular Characterization of Notch1 Positive Progenitor Cells in the Developing Retina

doi: 10.1371/journal.pone.0131054

Figure Lengend Snippet: Functional annotation for the selected genes revealed by One-Way ANOVA.

Article Snippet: To isolate Notch1 + cells, we used monoclonal biotin conjugated anti-Notch1 mouse antibodies (130-096-557, Miltenyi Biotec, Auburn, CA) and followed a protocol for immunomagnetic separation provided by Miltenyi Biotec (Auburn, CA).

Techniques: Functional Assay

Journal: PLoS ONE

Article Title: Molecular Characterization of Notch1 Positive Progenitor Cells in the Developing Retina

doi: 10.1371/journal.pone.0131054

Figure Lengend Snippet: For each gene, results are expressed as percentages ± SEM of the corresponding values in the Notch1 + cells isolated from E14 developing retinas (*P < 0.05).

Article Snippet: To isolate Notch1 + cells, we used monoclonal biotin conjugated anti-Notch1 mouse antibodies (130-096-557, Miltenyi Biotec, Auburn, CA) and followed a protocol for immunomagnetic separation provided by Miltenyi Biotec (Auburn, CA).

Techniques: Isolation

Journal: PLoS ONE

Article Title: Molecular Characterization of Notch1 Positive Progenitor Cells in the Developing Retina

doi: 10.1371/journal.pone.0131054

Figure Lengend Snippet: The expression of retinal cell markers in Notch1 + progenitor cells and in whole retina samples at E14 and P0.

Article Snippet: To isolate Notch1 + cells, we used monoclonal biotin conjugated anti-Notch1 mouse antibodies (130-096-557, Miltenyi Biotec, Auburn, CA) and followed a protocol for immunomagnetic separation provided by Miltenyi Biotec (Auburn, CA).

Techniques: Expressing

Journal: PLoS ONE

Article Title: Molecular Characterization of Notch1 Positive Progenitor Cells in the Developing Retina

doi: 10.1371/journal.pone.0131054

Figure Lengend Snippet: The putative members of E14 Notch1 and P0 Notch1 gene networks.

Article Snippet: To isolate Notch1 + cells, we used monoclonal biotin conjugated anti-Notch1 mouse antibodies (130-096-557, Miltenyi Biotec, Auburn, CA) and followed a protocol for immunomagnetic separation provided by Miltenyi Biotec (Auburn, CA).

Techniques:

Journal: PLoS ONE

Article Title: Molecular Characterization of Notch1 Positive Progenitor Cells in the Developing Retina

doi: 10.1371/journal.pone.0131054

Figure Lengend Snippet: List of RT-PCR primers.

Article Snippet: To isolate Notch1 + cells, we used monoclonal biotin conjugated anti-Notch1 mouse antibodies (130-096-557, Miltenyi Biotec, Auburn, CA) and followed a protocol for immunomagnetic separation provided by Miltenyi Biotec (Auburn, CA).

Techniques: